Serum adenylate kinase activity and isoenzyme in acute cerebral vascular disease.
نویسندگان
چکیده
منابع مشابه
Serum Adenylate Kinase Activity and Isoenzyme in Acute Cerebral Vascular Disease
Serum adenylate kinase (AK) activity was measured in cerebral vascular diseases and the AK isoenzyme pattern was studied in the patients suffering from cerebral infarction, cerebral hemorrhage, myocardial infarction, and polymyositis. There was a significant difference in AK activity between the control subjects and patients with acute cerebral infarction. Serum AK activity showed a peak on the...
متن کاملMeasurement of brain-specific creatine kinase isoenzyme activity in serum.
We have modified the columti-chromatographic technique of Mercer (1) to produce a sensitive and rapid separation of serum CK2 and CK3, even at normal total serum CK activity (3).12 However, CKj was not separated from CK2. Here, we report a modification of our method such that all three CK isoenzymes are separated by stepwise elution from a mini-column of diethylaminoethyl-cellulose. CK1 has onl...
متن کاملAnion-exchange chromatography of erythrocytic and muscle adenylate kinase and its effect on the serum creatine kinase isoenzyme assays.
We determined the elution profile of erythrocytic and muscle adenylate kinases (EC 2.7.4.3) in the Roche chromatographic creatine kinase procedure and studied the interference these enzymes would cause in the isolation and assay of serum creatine kinase (EC 2.7.3.2) isoenzymes. Both adenylate kinases co-elute with the creatine kinase MM fraction and do not interfere with the isolation or assay ...
متن کاملMass concentration and activity concentration of creatine kinase isoenzyme MB compared in serum after acute myocardial infarction.
We compared three current methods (immunoinhibition, "Isomune-CK" immunoprecipitation, and the Tandem-E CKMB II immunoenzymometric assay) for determination of creatine kinase (CK; EC 2.7.3.2) isoenzyme MB in serum. Although results inter-correlated well, the immunoinhibition assay gave higher activity values. Atypical CK forms did not interfere with the immunoprecipitation and immunoenzymometri...
متن کاملCreatine kinase isoenzyme activity in human placenta and in serum of women in labor.
Creatine kinase (EC 2.7.3.2) isoenzymes in extracts of human placenta and in serum from nonpregnant women and women in labor were separated on columns containing diethylaminoethyl-cellulose and assayed. The distribution of the isoenzymes in placenta (n = 10) was 80% BB (200 +/- 66 U/g (wet weight), 19% MM (49 +/- 30 U/g), and 1% MB (2.6 +/- 1.7 U/g); The geometric mean for the serum BB activity...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Biochemistry and Nutrition
سال: 1989
ISSN: 1880-5086,0912-0009
DOI: 10.3164/jcbn.7.9